生物通

STS-PCR与RFLP在伊朗人芽囊原虫检测和分型中的直接比较

Blastocystis是一种遗传多样性较高的单细胞真核肠道寄生虫，目前尚无金标准的诊断方法。本研究在伊朗进行，首次直接比较了序列标记位点（STS）PCR和限制性片段长度多态性（RFLP）在人类诊断中的效果。研究基于我们之前对同一样本群体的研究结果，旨在通过 ...
Nature

Novel mutations in the cytochrome P450 2C19 gene: a pitfall of the PCR-RFLP method for ...

CYP2C19 is a clinically important enzyme involved in the metabolism of therapeutic drugs such as (S)-mephenytoin, omeprazole, proguanil, and diazepam. Individuals can be characterized as either ...
生物通

基于限制性片段长度多态性(RFLP)的牛冠状病毒快速基因分型新方法 ...

为解决传统RT-PCR/RFLP方法对部分日本牛冠状病毒(BCoV)流行株基因分型失效的问题，日本研究人员通过引入AvaII、EcoO65I、AfaI和 ...
Nature

Comparison of multilocus RFLPs and PCR-based marker systems for genetic analysis of the ...

The utility of multilocus RFLPs and three PCR-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter-Simple Sequence Repeat-PCR (ISSR-PCR) and simple sequence repeats (SSRs) for genetic ...