A collection of protocols covering a variety of methods to achieve super resolution in microscopy to include structured illumination, single-molecule fluorescence and localization-based approaches.

Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...

When trying to measure molecular structures with nanometer precision, every bit of noise shows up in the data: someone walking past the microscope, tiny vibrations in the building and even the traffic ...

eDL-cSIM: An AI-driven super-resolution imaging method that captures high-quality live-cell dynamics in a single exposure, enhancing speed, resolution, and environmental robustness for advanced ...

Confocal microscopy is a specialized fluorescence imaging technique that scientists use to acquire images at greater resolution than conventional microscopy. 1 In addition to scanning the lateral x ...

To unravel the complexities of biological phenomena, scientists have long relied on microscopy to visualize the intricate details of their specimens, including tissue architecture, cell morphology, ...

Both images show individual red blood cells. The one on the right, produced by STED, more clearly shows the Piezo1 proteins (in green), which are a mechanical calcium channel and play a crucial role ...

When single-molecule super-resolution microscopes were first commercialized some 15 years ago, they made headlines for their ability to resolve individual molecules and structures at the nanometer ...

Researchers have built a tiny, lightweight microscope that captures neuron activity with unprecedented speed that can be used in freely moving animals. The new tool could give scientists a more ...