Gene therapy has often been described as a “black box” technique; that is, it is not known what happens to the cell after vector-mediated gene transfer. Although expression levels of the transgene are ...

Although prostate cancer initially responds and regresses in response to androgen-depletion therapy, most human prostate cancers will re-grow as an androgen-independent tumour. The goal of our study ...

The Nottingham Prognostic Index (NPI) applied to 467 patients (pts) with a long-term follow-up that were treated in a single institution No significant financial relationships to disclose. This is an ...

Serial analysis of gene expression (SAGE) uses mRNA from a sample to create complementary DNA (cDNA) fragments for amplification and sequencing using high-throughput sequencing technology. Credit: ...

Yes, many are attracted to next-generation sequencing, but loyalty to chip-based phenotyping has its rewards, particularly in screens of large sample sets. The microarray, the legacy technology for ...

In each type of cell, like a muscle cell or a skin cell, different genes are expressed (turned on) or silenced (turned off). If the cells that are turned on mutate, they could—depending on what role ...

The main steps involved and limitations of microarray analysis will be encountered. Two stages can be distinguished in microarray experiments: a pre-microarray experiment phase (tissue handling) and ...

No significant financial relationships to disclose. This is an ASCO Meeting Abstract from the 2004 ASCO Annual Meeting. This abstract does not include a full text component.

For 20 years, DNA microarrays have been the go-to method for revealing patterns of gene expression. But as the price of next-generation sequencing continues to fall, RNA sequencing (RNA-seq) has ...