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生物通
6 年
INTEGRATE!升级版转座子CRISPR-Cas系统电镜图揭示如何精准插入大片段DNA
哥伦比亚大学的研究团队在霍乱弧菌中发现了一个独特的“跳跃基因”(转座子)后,开发了一种名为INTEGRATE的工具,可以在基因组中精准位置插入大片段基因而不引入DNA断裂。对于侧重于敲除和降解目标DNA、且屡受到脱靶困扰的CRISPR技术,这种新的、精准插入 ...
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