本研究针对冷冻浆果中人类诺如病毒(HuNoV)检测依赖qRT-PCR技术无法区分病毒活性的问题,以鼠诺如病毒(MNV)为替代模型,通过ISO 15216:2017方法系统分析病毒载量与感染性的关联。结果表明qRT-PCR检测灵敏度(1.0 log PFU/25 g)较细胞培养法(3.1 log PFU/25 g)高2个 ...
COVID-19 is commonly diagnosed by quantitative fluorescence real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) (1, 2). The test is the sequential action of two enzymes–an ...