1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an interaction is ...

I’ve been working with computers for ages, starting with a multi-year stint in purchasing for a major IBM reseller in New York City before eventually landing at PCMag (back when it was still in print ...