What is a restriction map and how is it produced?... | Filo

(2) Your employer asks you to construct a restriction map of a ... | Filo

SOLVED: Texts: A restriction map lists the locations of DNA sequences that are cut by a particular restriction enzyme for a piece of DNA, such as a chromosome or a plasmid. Restriction maps are important when generating a construct for experimental use. Digesting the DNA sequence with the restriction enzymes will result in fragmented DNA of predictable sizes, based on the restriction map, that allow a researcher to analyze if his or her construct was generated correctly when visualized using gel

a. Given the following restriction map of a cloned 10 kb piece of DNA, what size fragments would you see after digesting this linear DNA fragment with each of the enzymes or combinations of enzymes listed? (1) EcoRI, (2) BamHI, (3) EcoRI + HindIII, (4) BamHI + Pstl, and (5) EcoRI + BamHI. b. What fragments in the last three double digests would hybridize on a Southern blot with a probe made from the 4 kb Bam HI fragment? | Numerade