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💐 Mixing Test Procedure (for prolonged PT or aPTT only) 💐 (Mixing Test : used to evaluate factor deficiency or the presence of inhibitors in coagulation.) 1. Prepare patient sample 🔸 Use plasma from a 3.2% Sidium citrated tube. 2.Prepare normal plasma (NPP) 🔸 Use pooled normal plasma. 3.Mix the plasma 🔸 Combine patient plasma and normal plasma in a 1:1 ratio. 4. Incubate at 37 °C 🔸 Some steps may require incubation for 1–2 hours. 5.Measure coagulation 🔸 Perform PT or aPTT. 6.Calculation f