Biochemical test reaction interpretation of Gram negative bacterium: TSI
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Biochemical test reaction interpretation of Gram negative bacterium: TSI
The most common routine tests used for biochemical test reaction for gram negative rods are Triple Sugar Iron(TSI) agar, Sulphide Indole Motility(SIM) test
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MICROBIOLOGY DIARY 🇳🇵 on Instagram: "TSI agar media: 🧪The biochemical test that measures the presence of metabolic pathway of the bacteria. 🧪TSI agar contains glucose, sucrose, lactose, ferric citrate and phenol red indicator in basal medium. 🧪The concentration of lactose and sucrose in the medium is ten times that of glucose. 🧪Ferric salt is added for testing H2s production. 🧪The medium is inoculated with bacterial culture using a straight wire, first stabbing the butt and then streaking
MICROBIOLOGY DIARY 🇳🇵 on Instagram: "TSI agar media: 🧪The biochemical test that measures the presence of metabolic pathway of the bacteria. 🧪TSI agar contains glucose, sucrose, lactose, ferric citrate and phenol red indicator in basal medium. 🧪The concentration of lactose and sucrose in the medium is ten times that of glucose. 🧪Ferric salt is added for testing H2s production. 🧪The medium is inoculated with bacterial culture using a straight wire, first stabbing the butt and then streaking
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API (Analytical Profile Index). There's different types of API techniques (API 20E (Enterobacteriaceae), API 20NE (non enterobacteriaceae), API 20 Staph. 20 referee to 20 biochemical tests. Before working API techniques, you must perform the following. 1-fresh cultures must be prepared (young colonies 18-24hr incubation) 2- Gram stain testing. 3- Oxidase test to differentiate between the positive Oxidase Pseudomonas aeruginosa and oxidase negative Enterobacteriaceae. 4 - API is used as a confirm
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